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doi: 10.1007/s12035-015-9337-5, PMID: [DOI] [PMC free article] [PubMed] [Google Scholar] 62.Louwerse ES, Weverling GJ, Bossuyt PM, Meyjes FE, de Jong JM

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The enzyme reactions contained 50 μl of the diluted supernatant, 450 μl of 0.04 mol/L phosphate buffer, and 3.6 ml substrate (α-naphtyl acetate), which were mixed and incubated in a shaker for 30 min at 37°C